TPMT and DPD Polymorphisms: Efficient Screening Method for Indian Patients Considering Taking Thiopurine and 5-FU Drugs

TPMT and DPD Polymorphisms: Efficient Screening Method for Indian Patients Considering Taking Thiopurine and 5-FU Drugs

INTRODUCTION Development of DNA-based tests for TPMT/DPD polymorphisms can help clinicians and patients to make important decisions about cancer treatment. Also, due to lack of Indian data, we aimed at the development and validation of these tests in Indian patients. MATERIALS AND METHODS Molecular assays were used for identifying TPMT/DPD variations; validated by DNA sequencing. RESULTS Mo...

Should screening for DPD deficiency be mandatory before 5-FU exposure?

Are patients with intermediate TPMT activity at increased risk of myelosuppression when taking thiopurine medications?

UNLABELLED Thiopurine S-methyltransferase (TPMT) metabolizes thiopurine medications, including azathioprine and 6-mercaptopurine. Absent TPMT activity (i.e., in individuals homozygous for a variant TPMT allele) is associated with an increased risk of myelosuppression in patients taking thiopurine drugs. However, it is not clear if there is also an increased risk for patients with intermediate T...

Association of TPMT (rs1800460) Gene Polymorphism with Childhood Acute Lymphoblastic Leukemia in a Population from Guilan, Iran

Acute lymphoblastic leukemia (ALL) is a malignant transformation and proliferation of lymphoid progenitor cells in bone marrow and blood, which is mainly found in children. Thiopurine methyltransferase (TPMT) is a thiopurine drug metabolizer enzyme that is prescribed for the treatment of ALL. Several single nucleotide polymorphisms in the TPMT gene have been reported to be associated with the d...

Simple and Efficient Method for the Preparation of 5-Bromoisatins

Isatin, N-methyl isatin and N-benzylisatin were smoothly monobrominated in the 5-position by treatment with pyridinium bromochromate (PBC) in acetic acid medium. In the similar conditions indigo underwent bromination and oxidation to give 5-bromoisatin in high yield.